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Author Stott, Kristina, author.
Title Caulobacter Crescentus SpotT is essential for preventing the over-initiation of DNA replication during fatty acid starvation / by Kristina Stott.
Published [Northridge, California] : California State University, Northridge, 2012.
LOCATION CALL # STATUS
 Electronic Book  QH51 .Z95 2012 S76eb    ONLINE
  
Description 1 online resource (x, 81 pages)
Content Type text
Format online resource
File Characteristics text file PDF
Thesis M.S. California State University, Northridge 2012.
Bibliography Includes bibliographical references (pages 78-81).
Access Restricted access; Author's permission required.
Summary Caulobacter crescentus FabH is a cell cycle regulated -ketoacyl-acyl carrier protein synthase that initiates fatty acid elongation and is essential in rich PYE media. Depletion of FabH induces fatty acid starvation and a bacteriostatic condition in cells. Therefore, fatty acid synthesis serves as a checkpoint for cells that are about to begin cell division. Also, the accumulation of the master regulators DnaA, GcrA, and CcrM, is drastically reduced while CtrA accumulation is only slightly decreased. Although no significant changes are observed for gcrA transcription, dnaA transcription increases 2.5-fold and ctrA transcription increases 1.7-fold when FabH is depleted. Fatty acid starvation triggers the SpoT-dependent production of ppGpp, which presumably helps RNA polymerase to activate the transcription of genes that are beneficial under starvation conditions. Increased dnaA and ctrA transcription under fatty acid starvation is SpoT-dependent. In contrast, ccrM transcription decreases 2-fold under FabH-depletion conditions and is SpoT-independent. CtrA normally blocks the initiation of DNA replication, however, when FabH is depleted, and in the absence of SpoT, CtrA fails to accumulate; resulting in the buildup of multiple chromosomes. In sum, the miscoordination of cell cycle events, such as DNA replication, during FabH depletion, results from the absence of SpoT.
Note Description based on online resource; title from PDF title page (viewed on June 04, 2012).
Subject Fatty acids.
DNA replication.
Cell cycle -- Regulation.
Local Subject Dissertations, Academic -- CSUN -- Biology.
OCLC number 847714715